鸡源大肠杆菌耐药性分析及中药对大肠杆菌耐药性消除作用的研究

doi:10.11843/j.issn.0366-6964.2015.06.019

Abstract

Abstract:

The aim of this study was to study the drug resistance of chicken isolated Escherichia coli and role of traditional chinese medicines in the drug resistance elimination.Twenty kinds of E.coli isolated from chicken around Shanxi province were tested to determine the resistant rate to 22 kinds of antibiotic by Kirby-Bauer way，the plasmid analysis and transformation test were conducted.Based on the resistance test results to antibiotic，we also evaluated the elimination effects of several traditional Chinese medicine (TCM) extracts on the isolated chicken E.coli.The results showed that the percent of E.coli resistant to above 5 kinds of antibiotics reached 90%.The isolated E.coli exhibited the strongest resistant rate to Penicillin G (90%)，followed by Enrofloxacin (85%)，while exhibited the highest sensitivity to Colimycin and Furadantin.According to the plasmid analysis results，12 kinds of plasmid patterns in total were found.Plasmids collected from the near farms with each other were likely to have the similar patterns，while different types among diverse areas.The transformation test results showed that one plasmid could encode single or several resistance genes，and different plasmids could have same resistance gene as well.TCM extracts，especially Scutellaria baicalensis，had significant elimination function to the drug resistant E.coli.Majority of the TCM treated plasmids maintain the same patterns，while two plasmids change their profiles after treating with coptis chinensis and shuanghuanglian，losing two DNA bind respectively.After all，TCM extract treatment recovered the susceptibility of chicken E.coli to drugs，which support the permitted use of TCM on the chicken E.coli disease control.

CLC Number:

NING Guan-bao，NIU Yi-ru，ZHANG Ding，LI Hong-quan，MA Hai-li，GAO Rong-kun，HAO Wei-fang，GAO Wen-wei，ZHAO Yu-jun，GAO Shi-min，LI Gui-lan，LI Jian-hui，YAN Fang，TIAN Wen-xia. Studies on the Drug Resistance of Chicken Isolated Escherichia coli and Role of Traditional Chinese Medicines in the Drug Resistance Elimination[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(6): 1018-1025.

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Studies on the Drug Resistance of Chicken Isolated Escherichia coli and Role of Traditional Chinese Medicines in the Drug Resistance Elimination

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